Composite

Part:BBa_K3505035:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-20)


pAndersonJ23115:RBS-tyrosinase:AIDA-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1502
    Illegal EcoRI site found at 2198
    Illegal XbaI site found at 1106
    Illegal PstI site found at 1550
    Illegal PstI site found at 2136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1502
    Illegal EcoRI site found at 2198
    Illegal NheI site found at 11
    Illegal NheI site found at 34
    Illegal PstI site found at 1550
    Illegal PstI site found at 2136
    Illegal NotI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1502
    Illegal EcoRI site found at 2198
    Illegal BglII site found at 534
    Illegal BamHI site found at 517
    Illegal BamHI site found at 2400
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1502
    Illegal EcoRI site found at 2198
    Illegal XbaI site found at 1106
    Illegal PstI site found at 1550
    Illegal PstI site found at 2136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1502
    Illegal EcoRI site found at 2198
    Illegal XbaI site found at 1106
    Illegal PstI site found at 1550
    Illegal PstI site found at 2136
    Illegal AgeI site found at 596
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct is cloned in a1RBBa_K3505008